This dataset presents a palaeoecological record of stratigraphy, radiocarbon-dated chronology, spores of coprophilous fungi (SCF), pollen and charcoal from lake sediment cores collected from Laguna La Yeguada, Panama. Laguna La Yeguada is an elliptical lake situated in the Veraguas Province within the Isthmus of Panama (8°27' N, 80°51' W). The data were collected to investigate the Late Quaternary megafaunal extinction in Panama and establish the first detailed record of megafaunal presence/absence, vegetation dynamics, and fire activity, respectively, spanning the Late Pleistocene to the Holocene. The data was collected for the PhD research titled: Ecological consequences of Pleistocene megafaunal declines in the Neotropics (https://gtr.ukri.org/projects?ref=studentship-2859430), funded by https://gtr.ukri.org/projects?ref=NE/S007504/1. The data is to be published in the Quaternary Science Reviews Journal: Pym, F., Franco-Gaviria, F., Raczka, M., Adediran, G.A., Sitch, S. and Urrego, D.H. The Timing and Ecological Consequences of the Late Pleistocene Megafaunal Declines on the Isthmus of Panama: Implications for Trophic Rewilding, Quaternary Science Reviews.