This dataset contains measurements of ozone (O3) concentrations, and environmental conditions experienced by tropical tree species grown in a series of experiments using Open Top Chambers (OTC's) conducted between July 2020 and November 2022 This also includes information on final biomass and leaf-level functional traits of experimental plants. The species studied include Brachichyton acerifolius, Carallia brachiate, Calophyllum inophyllum, Chionanthus ramiflorus, Darlingia darlingiana, Flindersia pimenteliana, Homalanthus novo-guineensis, Inga edulis, Syzygium gustavioides, and Theobroma cacao. Environmental variables recorded included air temperature, relative humidity and photosynthetically active radiation, alongside O3 concentrations in each of nine OTC's. While harvested plants were analysed to determine total dry-biomass and biomass partitioning as well as leaf functional traits such as leaf mass per unit are, antioxidant capacity, total phenolic content and leaf nitrogen content. This dataset also contains the calculated phytotoxic O3 dose (POD) for each species calculated when employing the DO3SE model and parametrized function of stomatal conductance. This data was supported by the NERC project, NE/R001812/1: Ozone impacts on tropical vegetation; implications for forest productivity (Trop-Oz). Full details about this dataset can be found at https://doi.org/10.5285/10b70ce2-29ac-4676-a664-b6054b5ef650